The Effect of Conjugated Linoleic Acid (CLA) on Male Reproductive Hormones in Mice

Spermatogenesis is a physiological reaction dependent to harmony of gonadotropins and steroid hormones activities. Conjugated linoleic acid is a group of long chain unsaturated fatty acids of a conjugated bound which are found in dairy products, beef and lamb. There are strong reasons that g/kg of food affects mediators involved in spermatogenesis. The aim of this study was determination of effect of various CLA doses on hormones and systemic factors of various CLA doses on hormones and systemic and local factors which affect spermatogenesis. Fifty mature male mice were divided in five groups (T0: control, T1: placebo and T2-T4: treatment groups). Each group had two replications with four mice in each replication. Samples received control diet (0 g/kg of conjugated linoleic acid) or treatment diet (0.1, 0.3 and 0.5 g/kg of CLA) which was replaced with corn oil of diet. After 30 days, blood samples were taken and level of FSH, LH and testosterone hormones were measured. Data were analyzed using ANOVA method and SPSS program and means were compared using Duncan's multiple ranges test at 5% probability level. CLA increased testosterone, LH and FSH level significantly in T3 and T4 groups. According to results, CLA has significant effect on secondary sexual activity of male animal which can affect spermatogenesis rate.